Our laboratory is focused on providing tools and improving existing genetic transformation systems for vector mosquitoes. One of our approaches is the identification and incorporation of boundary elements, or insulators, into transposable element constructs to protect transgenes from position effects and positive effect variegation upon integration into the mosquito genome. An alternative approach is the development of site-specific integration systems through the use of chimeric transposases fused with highly specific DNA binding domains such that the site of integration in the mosquito genome can be controlled.